This video looks at gram staining: a common lab technique used to differentiate bacteria based on their cell walls (Gram Positive vs Gram Negative).
UJEMI Methods Papers and Videos
Gram Staining
Wednesday, September 13, 2017
Volume 1
Fall 2016 / Winter 2017
Plating
Wednesday, September 13, 2017
Volume 1
Fall 2016 / Winter 2017
In this video we look at plating methods used in the laboratory.
Serial Dilutions
Wednesday, September 13, 2017
Volume 1
Fall 2016 / Winter 2017
This video looks at the theory and process of preparing serial dilutions and calculating colony counts.
Plasmid Preparation
Wednesday, September 13, 2017
Volume 1
Fall 2016 / Winter 2017
The first part of the video covers the general theory behind the extraction and isolation of plasmid DNA. After, we take a look at how this is done in the laboratory using a plasmid isolation kit.
Lysogeny Broth Agar Plates
Wednesday, September 13, 2017
Volume 1
Fall 2016 / Winter 2017
This video covers the step by step process of preparing Lysogeny Broth (LB) plates.
RNA Extraction Tutorial
Wednesday, August 16, 2017
Volume 1
Fall 2016 / Winter 2017
This video has two sections: In the first part, we explore some background theory on RNA preparation and in the second part, demonstrate the steps needed to successfully extract RNA from a sample.
Spot Plating Assay for the Determination of Survival and Plating Efficiency of Escherichia coli in sub-MIC Levels of Antibiotics
Thursday, July 13, 2017
Volume 1
Fall 2016 / Winter 2017
Spot plating is an alternative method to spread plating that generates more replicates in shorter periods of time.
Preparation of Calcium Competent Escherichia coli and Heat-Shock Transformation
Thursday, July 13, 2017
Volume 1
Fall 2016 / Winter 2017
Calcium chloride heat-shock transformation is a powerful molecular biology technique used to introduce foreign DNA into a host cell. The concept of the technique is to render cells competent using CaCl2 to allow for introduction of plasmid.
Phage Overlay Assay for Quantification of Infectious Bacteriophage Particles
Thursday, July 13, 2017
Volume 1
Fall 2016 / Winter 2017
The historical applications of plaque assays included isolation of virus and enumeration of viral particles in a solution.
Antisense RNA Insert Design for Plasmid Construction to Knockdown Target Gene Expression
Thursday, July 13, 2017
Volume 1
Fall 2016 / Winter 2017
Regulatory RNA molecules are common tools used in bacterial gene regulation. This paper focuses on the steps in designing an antisense RNA component for insertion into a plasmid in order to silence a gene in the plasmid host cell.