Phage Overlay Assay for Quantification of Infectious Bacteriophage Particles


Francis, Freddy; Miller, Rachel; Nagra, Manveer​

Volume 1
Fall 2016 / Winter 2017

The historical applications of plaque assays included isolation of virus and enumeration of viral particles in a solution. Plaque assays quantify viral particles by observing plaque forming units which are formed due to lytic viruses that lyse cells, and cells adjacent to them in a lytic life cycle within a confluent lawn of bacteria. We describe a method to prepare double agar overlay plaque assays, in an effort to quantify the viral particles in a solution.