Herein we describe a method used to effectively extract RNA from the Gram-negative bacterium Escherichia coli grown in Lysogeny Broth (LB). We employ a Proteinase K and enzymatic lysis step to facilitate the release of RNA from bacterial cells.
UJEMI Methods Papers and Videos
RNA extraction of Escherichia coli grown in Lysogeny Broth for use in RT-qPCR
Thursday, July 13, 2017
Volume 1
Fall 2016 / Winter 2017