Escherichia coli K30 produce a Type I (Colanic Acid) capsule which protects the bacterium from environmental stresses, including host immune responses. It is unknown how the presence of Type I capsule affects antibiotic resistance in the bacterium.
Current UJEMI Papers
Deletion of Escherichia Coli K30 Type I Capsule Assembly Gene wzc Confers Resistance to The Antibiotic Erythromycin in Solid Media
07/13/2017
Volume 21
Fall 2016 / Winter 2017
Ribosomal 16S rRNA Sequencing Shows That A Putative yidC Escherichia coli Knockout Strain Is Actually Rhizobium
07/13/2017
Volume 21
Fall 2016 / Winter 2017
To study the function of the major periplasmic domain of the inner membrane insertase YidC, Choi et al. (JEMI, Volume 20) constructed two chromosomal yidC knockout strains of Escherichia coli, WJKC-1 and WJKC-2.
Use of Matrix Assisted Laser Desorption/Ionization Time-of- Flight Mass Spectrometry (MALDI-TOF-MS) for Detection of Escherichia coli in Clinical Urine Samples
07/13/2017
Volume 21
Fall 2016 / Winter 2017
Urinary tract infections (UTIs) are very common and are becoming a concern in the medical community due to the growing prevalence of antimicrobial resistance in causative pathogens.
Role of the Rcs Phosphorelay in Intrinsic Resistance to Penicillin, Phosphomycin, and Cefsulodin in Escherichia coli K12
07/13/2017
Volume 21
Fall 2016 / Winter 2017
The Regulator of Capsule Synthesis (Rcs) of Escherichia coli detects cell wall stress and elicits a stress response. The Rcs phosphorelay system consists of the outer membrane kinase sensor RcsF, the inner membrane kinases RcsC and RcsD, and then response regulator RcsB.
Investigation of Wza in Erythromycin Sensitivity of Escherichia coli K30 E69 by Genetic Complementation
07/13/2017
Volume 21
Fall 2016 / Winter 2017
Escherichia coli K30 strains express group 1 capsule that is polymerized and transported by the Wzy-dependent pathway. This involves proteins encoded by genes wza, wzb, and wzc.
Reduced Bacteriophage T7 Infectivity of Gentamicin Treated Escherichia Coli UB1005 Is Likely Not a Result of Treatment- Induced Release of Cellular Factors Into The Culture Supernatant
07/13/2017
Volume 21
Fall 2016 / Winter 2017
Pre-treatment of Escherichia coli UB1005 with sub-lethal concentrations of gentamicin has been shown to reduce the infectivity of Bacteriophage T7. However, levels of infectivity has been shown to increase after a wash following antibiotic treatment.
Development of a Microtiter Plate Assay for Real Time Analysis of T7 Bacteriophage Mediated Lysis of Escherichia coli
07/13/2017
Volume 21
Fall 2016 / Winter 2017
When stressed, Escherichia coli releases soluble factors such as outer membrane vesicles. The release of these factors in the extracellular milieu has been shown to prevent bacteriophage infection in some cases.
Development of a Plasmid-Based System for Studying DNA Repair Mechanisms in Escherichia coli
07/13/2017
Volume 21
Fall 2016 / Winter 2017
Nucleotide excision repair is a DNA repair mechanism involved in the repair of ultraviolet radiation damage. The key proteins in this system are the four Uvr proteins: UvrA, UvrB, UvrC, and UvrD.
The Rcs-Phosphorelay Pathway Is Not Essential for Intrinsic Antibiotic Resistance to β-lactam Antibiotics in Escherichia coli
07/13/2017
Volume 21
Fall 2016 / Winter 2017
Rcs phosphorelay pathway in Escherichia coli is a response system that senses outer membrane stress conditions and regulating capsule and biofilm formation which can result in antibiotic resistance.
Soluble LPS May be a More Potent Competitive Inhibitor to Polymyxin B Than Escherichia coli O9a:K30 Capsular Polysaccharides
07/13/2017
Volume 21
Fall 2016 / Winter 2017
Escherichia coli expresses an extracellular layer of capsular polysaccharide (CPS) that confers protection from environmental stressors such as desiccation and antibiotics.